Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 13th International Conference on Metabolomics and Systems Biology Hilton Zurich Airport Hohenbuehlstrasse 10, 8152, Opfikon, Switzerland.

Day 2 :

OMICS International Euro Metabolomics 2018 International Conference Keynote Speaker Mikhail Y Golovko photo
Biography:

Mikhail Y Golovko obtained his PhD at Tver State Medical University, Russia. He is currently an Associate Professor in the Department of Biomedical Sciences, University of North Dakota (UND), USA.He joined UND in 2003 post completion of his PhD. His research interests are focused on brain lipid metabolism under neurodegenerative conditions including Alzheimer's disease and stroke.

 

Abstract:

A number of tissue bioactive lipids, including eicosanoids, are dramatically and instantly increased during tissue removal from the body and handling, thus altering quantification results for their basal levels. In addition to enzymatic synthesis, eicosanoid like isoprostanes are also produced upon oxidative stress. We, and others, have demonstrated that high energy focused microwave irradiation (MW) prevents a rapid, 30-fold increase in brain and 150-fold increase in kidney eicosanoid mass within seconds upon tissue extraction from the body. In addition, MW is also required to prevent postmortem alterations of another bioactive lipid group, endocannabinoid, including 2-arachydonoylglycerol (2-AG) and N-arachidonoylethanolamine. We validated stability of endogenous eicosanoids and endocannabinoids under tissue exposure to MW and the application of MW to measure true basal levels of prostaglandins, isoprostanes, and endocannabinoids. We also demonstrated that chiral chromatography is required to differentiate between eicosanoids and non-enzymatically produced isoprostanes. Our results indicate that MW combined with chiral LC-MS/MS is a safe and required technique to quantify tissue levels of a number of bioactive lipids including eicosanoids.

Break: Networking & Refreshments 10:45-11:05 @ Europa Foyer

Keynote Forum

Eloiza H Tajara

University of Sao Paulo, Brazil

Keynote: Plasma metabolomics for identifying diagnostic and prognostic biomarkers of oral cancer

Time : 11:05-11:50

OMICS International Euro Metabolomics 2018 International Conference Keynote Speaker Eloiza H Tajara photo
Biography:

Eloiza H Tajara obtained her Bachelor’s Degree in Biological Sciences (1969) and PhD Degree in Genetics (1980) at the University of São Paulo, Brazil. She did her Postdoctoral studies at the Southwest Biomedical Research Institute (Arizona, USA) and was a Fellow of the Union for International Cancer Control in the Eleanor Roosevelt Institute for Cancer Research (Colorado, USA) in 1994 and in the University of Chicago (USA) in 1997. She is currently an Associate Professor at the Faculty of Medicine of São José do Rio Preto, Sao Paulo, Brazil. She is the Coordinator of GENCAPO (Head and Neck Genome Project), a consortium of research groups from universities and hospitals in the State of São Paulo, in collaboration with researchers from the Federal University of Espírito Santo and the International Agency for Research on Cancer, Lyon, France whose goal is to develop clinical, epidemiological and molecular analysis of head and neck cancer. She has experience in Genetics and Molecular Biology, with emphasis on Cancer. Her scientific papers were cited 3435 times and the H index is 28.

Abstract:

Metabolomics has proved to be an important tool in cancer research, it allows the identification of molecular pathways behind the tumor phenotype and clinically useful markers. In fact, the data have shown the potential of metabolites to classify different types of cancer, both in diagnostic and prognostic levels. In head and neck cancer, several metabolomic data have been published in biofluids and tumor tissues. In the present study, plasma analysis was performed to identify metabolic profiles in oral squamous cell carcinoma (OSCC) patients and controls as well as in patients at different stages of disease. 1H NMR (Proton Nuclear Magnetic Resonance) analysis was performed on plasma samples from 47 OSCC cases and 49 controls to investigate association of metabolite concentrations with clinical, pathological and lifestyle variables. The results showed that clinical and lifestyle variables affect the plasma concentration of metabolites, especially those involved in metabolic pathways related to energy homeostasis. Mass spectrometry analysis of plasma samples was also performed to compare metabolite concentrations of 61 OSCC patients with 61 controls and of different cancer stages to obtain an insight on the dynamics of metabolite changes during oral neoplastic progression. Identification of the metabolites with significantly different concentrations was carried out using the PLS-DA (Partial least squares Discriminant Analysis) method. The 15 top features with higher VIP scores included acylcarnitine, serotonin and phosphatidylcholines more expressed in plasmas from patients. In addition, the data showed a deregulation in
carnitine concentrations and an increased biosynthesis of unsaturated lipids during neoplastic transformation, but more evident in the stage with larger tumor volume. In summary, the OSCC patients exhibited a distinct plasma metabolic profile suggestive of abnormal lipogenesis and energy metabolism, which is apparent in advanced stages of the disease and may contribute to proliferation and inflammation. Such signature, if used in monitoring tests, may contribute to prognosis and treatment of OSCC.