15th International Conference on Metabolomics & Systems Biology
Alexandria University, Egypt
Title: Cellulose membranes are more effective in holding back vital proteins and exhibit less interaction with plasma proteins during hemodialysis
Biography: Marwa Eltoweissy
The vast majority of patients with end-stage renal disease are treated with intermittent hemodialysis as a form of renal replacement therapy. To investigate the impact of hemodialysis membrane material on vital protein removal, dialysates from 26 well-characterized hemodialysis patients were collected 5 min after beginning, during 5 h of treatment, as well as 5 min before ending of the dialysis sessions. Dialysis sessions were performed using either modiﬁed cellulose (n=12) (low-ﬂux and high ﬂux) or synthetic Polyﬂux (n=14) (low-ﬂux and high-ﬂux) dialyzer. Protein removal during hemodialysis was quantiﬁed and the dialysate proteome patterns were analyzed by 2-DE-MS and Western blot. There was a clear correlation between the type of membrane material and the amount of protein removed. Synthetic Poly ﬂux membranes exhibit strong interaction with plasma proteins resulting in a signiﬁcantly higher protein loss compared to modiﬁed cellulosic membrane. Moreover, the proteomics analysis showed that the removed proteins represented different molecular weight range and different functional groups: transport proteins, protease inhibitors, proteins with role in immune response and regulations, constructive proteins and as a part of HLA immune complex. The effect of this protein removal on hemodialysis treatment outcome should be investigated in further studies.