Metabolomics

It is the large-scale study of small molecules, commonly known as metabolites, within cells, bio fluids, tissues or organisms. Collectively, these small molecules and their interactions within a biological system are known as the metabolome. Metabolomics represents the molecular phenotype. Four conceptual approaches in metabolomics - target analysis, metabolite profiling, metabolomics, and metabolic fingerprinting. Target analysis includes the determination and quantification of a small set of known metabolites (targets) using one of the specific analytical technique of best performance. On the other hand Metabolite profiling aims at the analysis of a larger set of compounds, both known and unknown with respect to their chemical nature. This approach has been applied for many different biological systems using GC-MS, including plants, microbes, urine, and plasma samples. Metabolomics employs complementary analytical methodologies, for example, LC-MS/MS, GC-MS, and/or NMR, in order to determine and quantify as many metabolites as possible, either identified or unknown compounds. The last approach is metabolic finger-printing.
 
 

  • Metabolomics databases
  • Software workflow for metabolomics: Targeted and non targeted screening and validation
  • Metabolite identification and analysis

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